Figure 3.

Interaction between miR‐211 and TGFβR2 in NK-2 cells. (a) The predicted site in TGFβR2 3′‐UTR for binding miR‐211. (b) Expression of miR-211 was detected in NK-2/miR‐211 inhibitor cells or NK-2/miR‐NC cells by qRT-PCR assay. (c) Luciferase reporter assay of NK-2 cells transfected with the TGFβR2 3′‐UTR‐WT or TGFβR2 3′‐UTR‐M in the binding sites. Differences were observed when miR‐211 inhibitor was added, and miR‐NC was used as the control. (d) TGFβR2 protein was detected by western blot assay in NK-2 cells transfected with miR‐211 inhibitor or miR‐NC. (e) miR‐211 inhibitor could reverse the TGFβR2 protein expression by TGFβR2 siRNA transfection by Western blot analysis. Data shown are means ± SE for n = 3 independent experiments. Student’s t test,*P < 0.01