Figure 2.

High glucose inhibited osteogenic differentiation in MC3T3-E1 cells. osteoblast differentiation was induced in cells, and cells were treated with HG for 14 days. (a) ALP activity was measured with the kit. (b) Treated cells were stained with Alizarin Red and quantified accordingly. Scale bar = 50 μm. (c) The level of miR-340-5p was detected by qRT-PCR. (d) OCN, collagen-I, and RUNX2 protein levels were assessed with immunoblotting. OD, osteogenic differentiation; HG, high glucose; IOD, integrated optical density. Results were presented as means ± SD (N = 3 in each group). ^$$$P < 0.001 versus control group; ^&&P < 0.01 versus OD group