Figure 3.

Down-regulation of miR-340-5p promoted osteogenic differentiation in MC3T3-E1 cells. (a) Cells were infected with lentivirus-based miR-340-5p inhibitor (anti-miR-340-5p), pre-mir-340 (miR-340-5p) or their negative controls (anti-NC and NC) for 72 h. Subsequently, osteoblast differentiation was induced, and cells were treated with HG for 14 days. (b) ALP activity was measured with the kit. (c) Treated cells were stained with Alizarin Red and quantified accordingly. Scale bar = 50 μm. (d) OCN, collagen-I, and RUNX2 protein levels were assessed with immunoblotting. NC, negative control. Data were expressed as means ± SD (N = 3 per group). ^aaaP < 0.001 versus NC group; ^bbbP < 0.001 versus anti-NC group; ^&P < 0.05 and ^&&P < 0.01 versus OD group; ^!P < 0.05, ^!!P < 0.01 and ^!!!P < 0.001 versus OD+HG+anti-NC group