Figure 4.

Up-regulation of miR-340-5p blocked osteogenic differentiation in MC3T3-E1 cells. MiR-340-5p was overexpressed (miR-340-5p) via lentivirus infection. Subsequently, osteoblast differentiation was induced, and cells were treated with HG for 14 days. (a) ALP activity was measured with the kit. (b) Treated cells were stained with Alizarin Red and quantified accordingly. Scale bar = 50 μm. (c) OCN, collagen-I, and RUNX2 protein levels were assessed with immunoblotting. Data were indicated as means ± SD (N = 3 per group). ⁺P < 0.05, ⁺⁺P < 0.01 and ⁺⁺⁺P < 0.001 versus OD+HG+NC group