Figure 3.

Targeting H19 enhances breast cancer sensitivity to Dox

MCF-7 cells were transfected with pCMV6 or pCMV6-H19, after 24 h, cells were treated with indicated 1.2 μM Dox for additional 72 h. (a) qRT-PCR analysis of relative H19 expression in MCF-7 cells; (b) Cell viability was detected by MTT assay; (c) Cell apoptosis was assessed by Annexin-V-FITC/PI staining assay by flow cytometry. MCF-7/Dox cells were transfected with NC siRNA or H19 siRNA, after 8 h, cells were treated with indicated 1.2 μM Dox for additional 72 h. (d) qRT-PCR analysis of relative H19 expression in MCF-7 cells; (e) Cell viability was detected by MTT assay; (f) Cell apoptosis was assessed by Annexin-V-FITC/PI staining assay by flow cytometry. *P < 0.01.