Figure 4.

Targeting H19 reduced cell viability and induction of apoptosis by PARP1 upregulation in Dox-treated breast cancer cells

MCF-7 cells were transfected or co-transfected with PARP siRNA, pCMV6-H19, pCMV6 – PARP1 and its controls and treated with Dox (1.2 uM) for 72 h. MCF-7/Dox cells were transfected or co-transfected with PARP siRNA, H19 siRNA, pCMV6-H19 and its controls and treated with Dox (1.2 μM) for 72 h. (a,e) PARP1 protein expression was analyzed by western blot assay; (b,f) PARP1 mRNA expression was analyzed by qRT-PCR assay; (c,g) Cell viability was detected by MTT assay; (d,h) Cell apoptosis was assessed by Annexin-V-FITC/PI staining assay by flow cytometry. *P < 0.01.