Figure 5.

Targeting H19 decrease resistance of breast tumor to Dox in xenograft tumor models

(a) MCF-7/Dox cells stably expressing H19 shRNA or NC shRNA were injected into nude mice. (b) MCF-7 cells stably expressing pCMV6-H19 or pCMV6 were injected into nude mice. When the tumor volume reached 100 mm³ (4 weeks), the mice (n = 8 per group) were, respectively, treated with PBS (0.1 mL, tail i.v. injection), Dox (0.1 mL, 10 mg/kg, tail i.v. injection, 4 times/week) and housed for another 24 consecutive days. Tumor volume was measured once per three days by using calipers (as indicated at each time point) for 24 days. (c, e) H19 mRNA expression was analyzed by qRT-PCR assay; (d,f) PARP1 mRNA expression was analyzed by qRT-PCR assay; (g,h) PARP1 protein expression was analyzed by western blot assay. *P < 0.01.