Figure 6.

E2F6 was a target of miR-197-3p in CC cells. (a) The binding sites between E2F6 and miR-197-3p were predicted. (b) Luciferase activity was analyzed in HeLa cells co-transfected with E2F6-WT+NC mimic, E2F6-WT+miR-197-3p mimic, E2F6-MUT+NC mimic, and E2F6-MUT+miR-197-3p mimic. (c) E2F6 expression was measured in CC tissues and paired normal tissues via RT-qPCR. (d) The correlation between E2F6 and miR-197-3p was analyzed by Pearson’s correlation analysis. (e) Via RT-qPCR, E2F6 expression was measured in HeLa cells transfected with sh-NC, sh-circ-ZNF609, sh-circ-ZNF609+ NC inhibitor, and sh-circ-ZNF609+ miR-197-3p inhibitor. *p< 0.05