Figure 3.

Dose optimization of lead inhibitors identified from the screen for enhancing transgene expression in CHO-K1 cells. A second plasmid, pGL3, which expresses luciferase protein under the control of the SV40 promoter was evaluated; the original screening plasmid (pEF-Luc) was also included. In addition, two polymers N-RDGE (top) and PG-C18 (bottom), were evaluated for delivering plasmid DNA. Transgene expression efficacies are reported normalized to the vehicle control (0.2% DMSO + polyplex and indicated by “0” on the x-axis, normalized to 1 on the y-axis). Cell viability, as measured using the MTT assay, are plotted on the secondary axes (dashed lines). * indicates p-value < 0.05, as determined using Student’s t-test, each condition relative to vehicle control + polyplex formed with same polymer/plasmid combination.