Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Acetylated tubulin | Thermo Fisher | Mouse monoclonal 32–2700 RRID:AB_2533073 |
IF(1:5000), WB(1:1000) |
| Antibody | ActinRed-555 | Molecular probes | R37112 | Manufacturer instructions |
| Antibody | Arl13b | Proteintech | Rabbit Polyclonal 17711–1-AP RRID:AB_2060867 |
IF(1:5000) |
| Antibody | AKAP220 | Santa Cruz Biotech | Goat polyclonal No longer available | IF (1:500) |
| Antibody | AKAP220 | Rockland | Rabbit polyclonal Custom generated | WB (1:500) |
| Antibody | Amersham ECL Mouse IgG, HRP-linked F(ab')₂ fragment (from sheep) |
GE Life Sciences |
NA9310 RRID:AB_772193 |
WB (1:10000) |
| Antibody | Amersham ECL Rabbit IgG, HRP-linked F(ab')₂ fragment (from donkey) |
GE Life Sciences | NA9340 RRID:AB_772191 |
WB (1:10000) |
| Antibody | Donkey anti-goat IgG-HRP |
Santa Cruz | sc-2020 RRID:AB_631728 |
WB (1:10000) |
| Antibody | Donkey anti-Mouse IgG, Alexa Fluor 488 | Invitrogen | A-21202 RRID:AB_141607 |
IF (1:500) |
| Antibody | Donkey anti-Mouse IgG, Alexa Fluor 555 | Invitrogen | A-31570 RRID:AB_2536180 |
IF (1:500) |
| Antibody | Donkey anti-Mouse IgG, Alexa Fluor 647 | Invitrogen | A-11126 RRID:AB_221538 |
IF (1:500) |
| Antibody | Donkey anti-Rabbit IgG, Alexa Fluor 488 | Invitrogen | A-21206 RRID:AB_2535792 |
IF (1:500) |
| Antibody | Donkey anti-Rabbit IgG, Alexa Fluor 555 |
Invitrogen | A-31572 RRID:AB_162543 |
IF (1:500) |
| Antibody | Donkey anti-Rabbit IgG, Alexa Fluor 647 | Invitrogen | A-31573 RRID:AB_2536183 |
IF (1:500) |
| Antibody | GFP | Rockland | 600-101-215 goat polyclonal RRID:AB_218182 |
WB (1:1000), IF (1:500) |
| Antibody | HDAC6 | Proteintech | 12834–1-AP Rabbit Polyclonal RRID:AB_10597094 |
IF (1:200) |
| Antibody | V5 | Invitrogen | R960-25 Mouse monoclonal RRID:AB_2556564 |
IF (1:500) |
| Cell line (H. sapein) | HEK293 | RRID:CVCL_0045 | Maintained in Scott lab in DMEM supplemented with 10% FBS | |
| Cell line (M. musculus) |
mIMCD3 | RRID:CVCL_0429 | Maintained in Scott lab in DMEM/F12 supplemented with 10% FBS | |
| Chemical compound, drug | DAPI | Thermo Fisher | 62248 RRID:AB_2307445 |
IF (1:1000) |
| Chemical compound, drug | Dimethylsulfoxide (DMSO) | Pierce | TS-20688 | Manufacturer’s instructions |
| Chemical compound, drug | DMEM FluoroBrite | Life Technologies | A1896701 | |
| Chemical compound, drug | DMEM/F-12 Hepes | Life Technologies | 11330057 | |
| Chemical compound, drug | DMEM, high glucose | Life Technologies | 11965118 | |
| Chemical compound, drug | Fetal Bovine Serum |
Thermo Fisher |
A3382001 | |
| Chemical compound, drug | Lipofectamine 2000 Transfection Reagent |
Invitrogen | 11668027 | |
| Chemical compound, drug | NuPAGE LDS Sample Buffer 4X | Thermo Fisher | NP0008 | |
| Chemical compound, drug | Opti-MEM, Reduced Serum Medium, no phenol red | Life Technologies |
11058021 | |
| Chemical compound, drug | ProLong Diamond Antifade Mountant |
Life Technologies |
P36961 RRID:SCR_015961 |
Manufacturer’s instructions |
| Chemical compound, drug | Polybrene | Santa Cruz | 134220 | Manufacturer’s instructions |
| Chemical compound, drug | Super Signal West Dura Extended Duration Substrate |
Thermo Fisher |
34075 | |
| Chemical compound, drug | TransIT-LT1 Transfection Reagent |
Mirus | MIR2300 | |
| Chemical compound, drug | Trypsin-EDTA (0.25%), phenol red | Gibco | 25200056 | |
| Commercial assay or kit | BCA Protein Assay Kit |
Thermo Fisher |
23227 | |
| Recombinant DNA reagent |
pMD2.G | RRID:Addgene_12259 | gift from Didier Trono; Addgene plasmid #12259 |
|
| Recombinant DNA reagent |
psPAX2 | RRID:Addgene_12260 | gift from Didier Trono; Addgene plasmid #12259 |
|
| Recombinant DNA reagent | Arl13b-GFP | RRID:Addgene_40879 | gift from Tamara Caspary; Addgene plasmid # 40879 | |
| Recombinant DNA reagent | lifeact-GFP | RRID:Addgene_58470 | gift from Dyche Mullins; Addgene plasmid # 58470 |
|
| Recombinant DNA reagent | pcDNA-HDAC6-flag | RRID:Addgene_30482 | gift from Tso-Pang Yao; Addgene plasmid # 30482 | |
| Recombinant DNA reagent | pEGFP-HDAC6-DC | RRID:Addgene_36189 | gift from Tso-Pang Yao; Addgene plasmid # 36189 | |
| Software, algorithm | Fiji/ImageJ | ImageJ (http://imagej.nih.gov/ij/) | ||
| Software, algorithm | GraphPad Prism |
GraphPad Prism (https://graphpad.com) |
||
| Software, algorithm | SoftWoRx | GE Healthcare | ||
| Other | Bolt 4–12% Bis-Tris Plus Gels | Invitrogen | NW04120BOX |