FIGURE 2.

STX2 promotes proliferation, migration and invasion of trophoblast cells. (A) qRT-PCR analysis and (B) western blot analysis of STX2 expressions in HTR-8/SVneo and primary human trophoblast cells transfected with Sh-STX2 and STX2-expressing lentivirus. (C) EdU assay and (D) colony forming assay of control and experimental cells in which STX2 was knocked down or overexpressed. Down-regulated STX2 reduced cell proliferation, whereas up-regulated STX2 increased cell proliferation. (E) Transwell assays of control and experimental cells in which STX2 was knocked down or overexpressed. Down-regulated STX2 inhibited cell migration and invasion, whereas up-regulated STX2 promoted cell migration and invasion. All the experiments were repeated three times independently. Data are represented as the mean ± SEM. Student’s t-test: **P < 0.01.