| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit monoclonal IgG anti-ACE2 (clone EPR4435(2)), dilution factor (1:150) | Abcam | Cat# ab108252; RRID: AB_10864415 |
| Rabbit IgG Isotype Control | Novus | Cat# NB810-56910; RRID: AB_844243 |
| Biotinylated goat anti-rabbit IgG (H+L) | Vector Laboratories | Cat# BA-1000; RRID: AB_2313606 |
| Biological samples | ||
| Human control organ donor duodenum FFPE blocks | nPOD repository https://www.jdrfnpod.org/ | Network for Pancreatic Organ Donors with Diabetes, RRID:SCR_014641 Cat# nPOD_6493, RRID:SAMN15879546 |
| Human control organ donor kidney FFPE blocks | nPOD repository https://www.jdrfnpod.org/ | Network for Pancreatic Organ Donors with Diabetes, RRID:SCR_014641 |
| Human control organ donor pancreas FFPE blocks | nPOD repository https://www.jdrfnpod.org/ | Network for Pancreatic Organ Donors with Diabetes, RRID:SCR_014641 Cat# nPOD_6493, RRID:SAMN15879546 |
| Human COVID-19 autopsy pancreas FFPE blocks | Department of Pathology, Louisiana State University | https://www.medschool.lsuhsc.edu/pathology/ |
| Chemicals, peptides, and recombinant proteins | ||
| Antibody diluent reagent | Life Technologies | Cat# 003118 |
| Normal goat serum | Vector Laboratories | Cat# H-1200 |
| Antigen Unmasking Solution, Citrate-Based | Vector Laboratories | Cat# H-3300-250 |
| Optik Hematoxylin | Avantik | Cat# R54576-A |
| Optik Aqueous Clarifier | Avantik | Cat# R54361-B |
| Optik Bluing Reagent | Avantik | Cat# R54363 |
| Xylene1 | Fisher Scientific | Cat# X3P-1gal |
| Ethanol 200 proof1 | Decon Labs | Cat# 2701G |
| Methanol1 | Fisher Scientific | Cat# A433P-4 |
| Hydrogen peroxide2 | Fisher Scientific | Cat# H325-500 |
| Citrate Buffer, pH 6.0 (10×) | Abcam | Cat# ab64214 |
| Shandon-Mount Mounting Media | Thermo Scientific | Cat# 1900331 |
| Critical commercial assays | ||
| H&E Staining Kit | Abcam | Ab245880 |
| Avidin/Biotin Blocking Kit | Vector Laboratories; www.vectorlabs.com | Cat# SP-2001 |
| ABC Elite standard detection kit | Vector Laboratories; www.vectorlabs.com | Cat# PK-6100 |
| ImmPACT DAB3 | Vector Laboratories; www.vectorlabs.com | Cat# SK-4105 |
| Software and algorithms | ||
| Aperio eSlideManager | Leica Biosystems | Version 12.4.0.5043 |
| Other | ||
| Microtome | n/a | n/a |
| Benchtop dry oven | n/a | n/a |
| Microwave oven, 600 Watt | n/a | n/a |
| Fume hood | n/a | n/a |
| Brightfield microscope | n/a | n/a |
| Aperio CS2 Slide Scanner (optional) | Leica Biosystems | n/a |
1CRITICAL: Flammable, highly toxic. Use only in a functioning fume hood. Wear gloves and a lab coat. Dispose of spent chemicals as hazardous waste.
2Strong oxidizer and can react with skin. Use only in a functioning fume hood. Wear gloves and a lab coat. Dispose of spent chemicals as hazardous waste, and avoid introducing any reagent to sink or water waste.
33,3′-Diaminobenzidine (DAB) is a known carcinogen, gloves and lab coat should be worn during use.