Figure 2.
Ectopic expression of G6PD impairs C. burnetii fitness during intracellular replication. The effect of complementing C. burnetii with zwf was tested under intracellular replication in physiologically diverse cell lines. (A) Replication of the parental strain (CbNMII) and CbNMII::Lp-zwf (Cb+G6PD) in J774A.1 cells. (B) Replication of CbNMII and Cb+G6PD in Vero cells. Data points represent the average of three independent experiments ± SEM. Asterisks indicate statistical significance (*P < 0.05; **P < 0.01; ***P < 0.001, unpaired Student's t-test).