Figure 3.

Coxiellaburnetii NADP⁺ and NADPH pools are affected by glucose availability. Intracellular pools of NADP⁺ and NADPH were measured in the parental strain (Cb_NMII) and CbNMII::Lp-zwf (Cb_+G6PD) following 4 days of replication in media containing (A) 0.5 or (B) 5.0 mM glucose. Each symbol represents a biological replicate. Open symbols indicate samples for which cofactor levels were below the level of detection. The data represent relative averages of three to four independent experiments ± SEM. Asterisks indicate statistical significance (*P < 0.05, paired Student's t-test).