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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Cancer Discov. 2021 Feb 15;11(7):1774–1791. doi: 10.1158/2159-8290.CD-20-1098

Figure 3.

Figure 3

(A) Representative IHC images of PTHrP staining in PanIN (from KC mice) or PDAC tumors (from KPC mice). Scale bars = 100μm.

(B) Western blots of PTHrP and ACTIN in cell lines isolated from WT pancreatic ductal epithelial cells (Pdx1-Cre; LSL-Rosa26YFP/YFP) or KPCY pancreatic cancer cells.

(C) Representative IF images of metastatic liver lesions from KPCY mice stained with YFP (green), PTHrP (red), and DAPI (blue). Scale bars = 100μm.

(D-F) (D) Representative brightfield dissection scope images of orthotopic injection tumors from KPCY-shNT, KPCY-shPthlh #1, and KPCY-shPthlh #2 cell lines 3-weeks post-implantation. (E) Quantification of pancreatic tumor burden normalized to body mass after orthotopic injection. (F) Quantification of the percentage of YFP+ metastatic tumor cells present in FACS analyzed livers in mice with implanted orthotopic primary tumors. Statistical analysis by Student’s unpaired t-test with significance indicated (*, p<0.05; error bars indicate SD; n=5 mice/group).