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. Author manuscript; available in PMC: 2021 Jul 21.
Published in final edited form as: Anal Chem. 2021 Jul 9;93(28):9845–9852. doi: 10.1021/acs.analchem.1c01433

Figure 5.

Figure 5.

(A) Relative quantification performance of 4-plex DiLeuPMP labeled O-glycans released from glycoprotein PSM. Labeled O-glycans were mixed at ratios of 1:1:5:10 and analyzed in triplicates. Box plots show the median (line), the 25th and 75th percentile (box), and the 5th and 95th percentile (whiskers); (B) Representative MS spectrum of reporter ion region for 4-plex DiLeuPMP labeled H2N3F; (C) LC retention times of reporter ions generated from 4-plex DiLeuPMP labeled H2N3F; (D) ESI-MS/MS fragmentation of DiLeuPMP labeled H1N2 from healthy human serum sample showing efficient fragmentation and abundant reporter ions in the zoomed inset allowing quantification. Note: H-hexose, N-N-acetylhexoamine, F-fucose.