Fig. 1.

LLC tumour growth and angiogenesis are reduced in PdgfrβCre + ;FAK^Y861F/Y861F mice. a PdgfrβCre + ;FAK^WT/WT, PdgfrβCre + ;FAK^Y397F/Y397F and PdgfrβCre + ;FAK^Y861F/Y861F mice were born at normal male;female ratios; b Mendelian ratios with c similar body weights. Pie chart in a represents percentage male:female ratio, in b represents % Cre + and Cre- mice born to each genotype (n = 60 mice/genotype). d Western blotting of primary pericytes isolated from PdgfrβCre + ;FAK^WT/WT, PdgfrβCre + ;FAK^Y397F/Y397F and PdgfrβCre + ;FAK^Y861F/Y861F mice confirmed reduced levels of p-Y397 and pY861-FAK in FAKY397F and FAKY861F pericytes, respectively. e pY861-FAK levels are significantly reduced in Y397F pericytes, n = 2 independent lysates/genotype. Bar chart represents mean pY861-FAK levels + sd. *P = 0.0155. GAPDH act as loading control. f pY416-Src levels are significantly reduced in Y397F-FAK pericytes. Blots shows pY416-Src, total Src and GAPDH loading control. g In vivo tumour growth was significantly reduced only in PdgfrβCre + ;FAK^Y861F/Y861F mice. Graphs represent mean tumour volume ± s.e.m.; n = 15 PdgfrβCre + ;FAK^WT/WT mice, 14 PdgfrβCre + ;FAK^Y397F/Y397F mice and 11 PdgfrβCre + ;FAK^Y861F/Y861F mice. ****P < 0.0001. Two-sided Mann–Whitney U rank sum test. h Tumour blood vessel density was significantly reduced only in PdgfrβCre + ;FAK^Y861F/Y861F mice. Bar charts represent mean blood vessel density + s.e.m. *P = 0.0498; ns, not significant; n = 6 PdgfrβCre + ;FAK^WT/WT tumours, 6 PdgfrβCre + ;FAK^Y397F/Y397F tumours and 5 PdgfrβCre + ;FAK^Y861F/Y861F tumours. Two-sided Student’s t-test. Representative endomucin stained LLC tumour sections are shown for each genotype. Scale bar, 50 μm