Fig. 7.

Supplementation of ATP reverses linalool-induced ERK phosphorylation independently of TRPM8 activation. a Western blot of p-ERK, ERK and β-actin expression in HDMECs, which were pretreated for 2 h with or without 5 µM AMTB or 1 mM ATP and then exposed for 30 min to 0 or 2 mM linalool. b Expression levels of pERK/ERK (in % of 0 mM linalool) as assessed by western blot (n = 3 independent experiments). c Intracellular ATP level (in % of 0 mM linalool) of HDMECs, which were treated with 0 or 2 mM linalool in the presence or absence of 5 µM AMTB or 1 µM PD0325901, as assessed by the luciferase assay (n = 4). d Ca²⁺ signal (F/F₀) in HDMECs, which were pretreated for 2 h with or without 1 µM PD0325901, loaded with Fluo-4 AM and then stimulated with 0 or 2 mM linalool. The onset of stimulation is indicated by a black arrow. e Peak amplitude (F/F₀) of Ca²⁺ signal in HDMECs, which were pretreated with or without PD0325901, loaded with Fluo-4 AM and then stimulated with vehicle or linalool. Means ± SEM. *P < 0.05 vs. 0 mM linalool. ^#P < 0.05 vs. 2 mM linalool