Fig. 3. ATM-3507 (ATM) synergistically induces apoptosis with vinorelbine (VNB) but not with paclitaxel (PTX).
a–f A2780, A2780cis and OVCAR4 were incubated with single agents or in combination as indicated for 48 h and the corresponding induction of apoptosis was measured by Annexin V apoptosis assay using flow cytometry. Dose–response curves of ATM, VNB and their combination are shown in a–c. Dose–response curves of ATM, PTX and their combination are shown in d–f. g–i Chou–Talalay analysis of synergy of two drug combinations in the three ovarian cancer cell lines. Lines showing the trend of CI values interpolated from actual CI values presented by each dot in the same drug combination. CI values <0.9 indicate synergy.24 Fraction affected indicates the percentage of induced apoptosis. Data were generated from at least three independent experiments and presented as mean ± S.D. if applicable.