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. 2021 May 12;125(2):265–276. doi: 10.1038/s41416-021-01420-y

Fig. 5. ATM-3507 (ATM) and both anti-microtubule agents, vinorelbine (VNB) and paclitaxel (PTX), enhance G1/S arrest with upregulation of p21 and p27.

Fig. 5

a The percentage of OVCAR4 cells that underwent different cell fates after completing their first mitosis. At least 85 cells from three independent experiments were analysed for each treatment. b Time cells spent in the next interphase. c Immunoblots and quantification of cyclin D1 and E1 in cell lysates from OVCAR4 cells treated with DMSO, ATM (5 μM), VNB (8 nM), A + V (5 μM ATM + 8 nM VNB), PTX (5 nM) and A + P (5 μM ATM + 5 nM PTX), respectively, for 36 h. Dead and mitotic cells were removed by shake-off. d Immunoblots and quantification of p16, p21 and p27 in cell lysates as in c. Data were collected from at least three independent experiments. Error bars are mean ± S.D.; *p < 0.05; **p < 0.001.