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. 2021 Jul 20;11:14809. doi: 10.1038/s41598-021-94236-y

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© The Author(s) 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Cryo-EM analysis of mHsp60. (a) A representative cryo-EM micrograph. Side and tilt views are highlighted by white and black arrowheads. (b) Representative reference-free 2D class averages images. (c) Cryo-EM reconstruction of the mHsp60 heptamer colored by local resolution. (d) A mHsp60 subunit displayed in cryo-EM reconstruction with local resolution and in ribbon representation. Ribbon color scheme, blue, green and red for the equatorial, intermediate and apical domains, is the same as Fig. 3C. (e) The EM density at the region involved in the inter-subunit interaction.