Table 2.
Cryo-EM data collection, refinement and validation statistics.
| Reconstruction data | mHsp60 (PSU COM) | mHsp60 (NCCAT) |
|---|---|---|
| Data collection information | ||
| Electron microscope | TFS Titan Krios | TFS Titan Krios |
| Operation voltage (kV) | 300 | 300 |
| Electron detector | Gatan K3 | Gatan K3 |
| Energy filter (slit width in eV) | 30 | no |
| Data collection software | TFS EPU | Leginon |
| Data collection mode | counting | Super-resolution counting |
| Nominal magnification | 120,000 × | 81,000 × |
| Pixel size in Å (super resolution) | 0.84 | 1.069 (0.535) |
| Total accumulated dose (e−/Å2) | 44 | 50–57 |
| Data process statistics | ||
| Box size (pixel) | 320 | 240 |
| Number of particles | 196,060 | 182,600 |
| CTF estimation | Gctf | CTFFIND4 |
| Data processing software | RELION 3.1 | RELION 3.1 |
| Symmetry imposition | C7 | C7 |
| B-factor applied (Å2) | − 151 | − 191 |
| Final resolution (Å) | 3.5 | 3.5 |
| Combined B-factor (Å2) | − 180 | |
| Combined resolution (Å) | 3.4 | |
| EMDB code | EMD-23217 | |
| Model refinement statistics | ||
| CCmasked (Model vs Data) | 0.73 | |
| CCvolume (Model vs Data) | 0.71 | |
| RMSD bond length (Å) | 0.007 | |
| RMSD bond angles (°) | 0.699 | |
| Ramachandran plot | ||
| Favored (%) | 88.85 | |
| Allowed (%) | 10.77 | |
| Outliers (%) | 0.38 | |
| Clash score | 12.51 | |
| PDB code | 7L7S | |