Fig. 3. Prostate cancer database expression data and patient microarray staining demonstrate significant EZH2 and NSD2 correlation within individual tumours and NSD2 and EZH2 expression predicts biochemical recurrence in PC.

a–c For individual tissue samples with expression for both enzymes available, mRNA expression values plotted for each marker. a TCGA (n = 491) consisting of hormone-sensitive primary PC. b MSKCC (n = 128) consisting of primary and metastatic PC samples. c SU2C (n = 270) consists of metastatic CRPC patients. RNA expression data were queried and downloaded using the cBioPortal platform. As sample distribution was found to be non-Gaussian, Spearman correlation coefficients were calculated. d, e Kaplan–Meier analysis performed using clinical outcome data available on cBioPortal for d TCGA (n = 498) and e MSKCC (n = 140). Progression-free time defined as time to biochemical recurrence as determined by PSA cut-offs. In each dataset, samples were assigned individual quartile rank for NSD2 and EZH2. Samples containing top quartile ranks for both NSD2 and EZH2 (n = 70; 21) were compared to all remaining samples in their respective cohorts (n = 428;119). P values are documented.