Skip to main content
. 2021 Jul 20;12:4427. doi: 10.1038/s41467-021-24724-2

Fig. 3. MARCH8 inhibits IAV replication in vivo.

Fig. 3

Six-week-old male C57Bl/6 mice were administered PBS, control (PPMO-NC), or MARCH8 targeting PPMO (PPMO-M8) intranasally for 2 consecutive days. On day 0, two or three mice per group were killed before infection. Fifteen mice per group were infected with PR8 virus (200 PFU) intranasally on day 0. Five mice per group were euthanized on days 3 and 6 post infection. Bronchoalveolar lavage fluid was collected to determine virus titer, and lungs were harvested to histopathology. In five mice per group, survival was studied until day 14 post infection. a Diagram shows experimental plan. b Graph shows mouse body weight ± SD (n = 5 mice) up to 10 days post infection, for at least five mice per group. c Graph shows mean lung virus titer ±SD (n = 5 mice) on days 3 and 6 post infection. d Mouse lungs were isolated on day 0 (before infection), day 3, and day 6 post infection. H&E staining was performed on lung sections. Scale bars represent 200 μm. Histological scoring is shown as mean ± SD (n = 4 mice), *P < 0.01, **P < 0.001, n.s., not significant, unpaired two-tailed Student t-test, without any adjustments for multiple comparisons.