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. 2021 Jul 20;4:887. doi: 10.1038/s42003-021-02418-1

Fig. 6. Mutant cycle analysis suggests that F232 and F279A interact in KCNQ1/KCNE1 channels but not in KCNQ1 channels alone.

Fig. 6

a If mutant 1 (green) and mutant 2 (orange) are functionally independent of each other, the sum of the free-energy changes of the two single mutants will be equal to the free-energy change of double mutant 1,2 (red). b If mutant 1 (green) and mutant 2 (orange) are functionally interacting with each other (indicated as a blue dashed line), the sum of the free-energy changes of the two single mutants will be not equal to the free-energy change of double mutant 1,2 (red). c Voltage dependence of current activation from KCNQ1+KCNE1 (black, n = 4), KCNQ1-F232A/KCNE1 (green, n = 4), KCNQ1-F279A/KCNE1 (orange, n = 3), and KCNQ1-F232A-F279A/KCNE1 (red, n = 5) channels. d Voltage dependence of current activation from KCNQ1 (black, n = 4), KCNQ1-F232A (green, n = 3), KCNQ1-F279A (orange, n = 4), and KCNQ1-F232A-F279A (red, n = 5) channels without KCNE1 association. The voltage protocol is indicated in Fig. 2. Data are shown as mean ± SEM.