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. 2021 Jul 20;7:184. doi: 10.1038/s41420-021-00570-5

Fig. 3. CIR induces premature senescence in isogenic HCT116 colorectal cancer cells depends on p21 but not p53.

Fig. 3

A Senescent cells were determined with β-galactosidase staining at day 1, 3, and 5. Scale bar: 20 µm. B Representative images of Ki67 distribution at the indicated time points. Scale bar: 20 µm. C Quantification of the mean number of Ki67 positive cells post-irradiation. The values of all experiments represent the mean ± standard error (SE) of three independent experiments performed in triplicate. D Senescence pathway activation mediated by kinetics of p21 and p16/pRb expression was measured by western blotting. The intensities of the protein expression bands were quantified against GAPDH from the untreated control cells and are presented as fold increases below the respective lanes. D represents Day. E The efficiency of p21 silencing was demonstrated by western blot analysis. NC‚ negative control. F Clonogenic survival of p53+/+ cells transfected by siRNA p21 (Si p21) or siRNA NC. 24 h after transfection, cells were irradiated at the indicated doses. Data represent the mean of three independent experiments in triplicate, and error bars represent standard error (SE).