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. 2021 Jul 20;12:4395. doi: 10.1038/s41467-021-24680-x

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© The Author(s) 2021, corrected publication 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Pattern diagram of sternum immobilisation by elastomer. b Surgical procedure of sternum immobilisation by elastomer in rat. c HE staining of rat sternum in sham, control and elastomer group. Masson staining of rat sternum in sham, steel wire and SHE group (blue part represented the deposition of collagen in sternum bone), scale bar = 500 μm. d Statistical scatter diagram of cortical bone thickness of rat sternum in sham, control, elastomer group (n = 5 in each group). e Statistical scatter diagram of collagen deposition of rat sternum in sham, control, elastomer group (n = 5 in each group). f Spinal X-ray imagination of porcine sternum (cross section) before and after immobilisation, respectively. High density shadows of sternum were marked with yellow arrows. Data were presented as mean ± s.d. Ordinary one-way ANOVA test with Tukey’s multiple comparisons test (d, e) was used for analysis of cortical bone thickness and quantity of collagen deposition. Cortical bone thickness (d): Sham compared to Steel wire, ns p = 0.7406; Sham compared to SHE, ns p = 0.4386; Steel wire compared to SHE, ns p = 0.8631; Quantity of collagen deposition (e): Sham compared to Steel wire, ****p < 0.0001; Sham compared to SHE, ****p < 0.0001; Steel wire compared to SHE, ns p = 0.3908. Source data are provided as a Source Data file. NS no significance.