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. 2021 Mar 3;80(8):1031–1039. doi: 10.1136/annrheumdis-2020-219649

Figure 6.

Figure 6

LAMP3 expression decreases NKCC1 expression in salivary gland epithelial cells. (A) Immunofluorescence images in murine submandibular glands (40× magnification) from mice treated with AAV2-LAMP3 or AAV2-GFP. (B) Expression area of NKCC1 was quantified in AAV2-GFP treated mice (N=4) and AAV2-LAMP3 treated mice 2 months (2M) post-cannulation (N=8) and 6 months (6M) post-cannulation (N=7). (C) Correlation between the NKCC1 and LAMP3 expression in submandibular gland specimens, or saliva flow. (D) Transcript change of Scl12a2 gene in submandibular gland tissues from AAV2-GFP treated mice (N=6) and AAV2-LAMP3 treated mice 2M (N=5) and 6M (N=9) post-cannulation. (E) Western blotting with indicated antibodies using acinar or ductal cell lysate 72 hours post-transfection with LAMP3 or empty plasmids. (F) Immunofluorescence images (40× magnification) of A253 cells 48 hours after transfection with LAMP3 or empty plasmids. Representative images from three independent experiments. Values are shown as the mean±SD. *p<0.05, **p<0.01, t-test with Bonferroni’s correction. LAMP3, lysosome-associated membrane protein 3; NKCC1, Na-K-Cl cotransporter-1.