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. 2021 Jul 7;8:704657. doi: 10.3389/fcvm.2021.704657

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Copyright © 2021 Pickel, Cruz-Garcia, Bandleon, Barkovits, Heindl, Völker, Abeßer, Pfeiffer, Schaaf, Marcus, Eder-Negrin, Kuhn and Miranda-Laferte.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In vivo induction of left ventriclulare hypertrophy produces a decrease in nucleus-targeted Ca_vβ₂. Western blot analyses (left panels) and bar plots of mean values of densitometry analyses from western blots (right panels) of: (A) left ventricle (LV) nuclear fractions, (B) LV membrane fractions and (C) right ventricle (RV) nuclear fractions. Per well, 50 μg of total proteins from sham-operated (N = 3) or TAC-operated (N = 3) mice were loaded and detected using an anti-Ca_vβ₂ antibody. Immunoblots to confirm the presence of the compartment-specific protein markers histone H3 (nucleus), Na⁺/K⁺ ATPase (membrane) and GAPDH (cytosol) were also performed. Ca_vβ₂ expression was normalized to histone-H3 (in A and C) or to Na⁺/K⁺ ATPase expression (in B). ^§§p < 0.05 vs. sham (two-tailed unpaired t-test).