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. 2021 Jul 20;9(7):e002319. doi: 10.1136/jitc-2020-002319

Figure 3.

Figure 3

p38 MAPK signaling acts as a compensatory survival pathway in M1 macrophages. (A) DigiWest phospho-protein pathway activity analysis, log2 Fc; non-parametric Wilcoxon rank-sum test (p<0.05). (B) Western blot analysis of mouse bone marrow-derived macrophages treated for 1 hour with GDC-0623 (500 nM) or DMSO before addition of stimuli as in figure 1 for 15 min. (C) Bar graphs of treated macrophage subsets with 10 µM BIRB 796, 100 nM GDC-0623, and a combination of 2 µM BIRB 796 and 100 nM GDC-0623. (D) Same experiment as in (C); depicted 1 µM GDC-0623. Mean±SEM, n=3. Two-way ANOVA with post hoc Dunnett test. Significance levels are indicated by asterisks (*p≤0.05; **p≤0.01; ***p≤0.001: ****p≤0.0001). ANOVA, analysis of variance; DMSO, dimethyl sulfoxide; MAPK, mitogen-activated protein kinase; M-CSF, macrophage-colony stimulating factor.