Figure 3.

EKTOMUN/SUREK induce less Fc-mediated cytotoxicity than ch14.18. (A) NB69 cells were seeded in the presence of CD16 positive Jurkat cells, which served as effector cells, at an E:T of 3:1. Ch14.18 and the GD2-directed trAb SUREK were added at rising concentrations. The binding of an antibody Fc-region to CD16 induces luciferase expression in Jurkat cells. The bioluminescent signal was measured 6 hours after adding the antibodies to the coculture. (B) The NB69 cell line was stably transduced with a GFP_ffluc construct and treated with rising concentrations of antibodies in the presence of 12.5% human serum from a healthy donor in the culture medium. Tumor cell lysis was determined by bioluminescent flux relative to untreated cells after 24 hours. Student’s t-test; n.s., not significant; ***p<0.001. huIgG, human immunoglobulin G.