FIGURE 3.

Functional annotation of genes modulated by tagatose in P. infestans. Differentially expressed genes (DEGs) were identified in P. infestans at 4 and 10 days after incubation (DAI) on pea agar medium in the presence of tagatose compared to the respective control samples grown in the absence of tagatose. Functional categories (A) were assigned based on the protein description of upregulated (solid bars) or downregulated (stripped bars) genes at both time points (4 and 10 DAI; orange) and exclusively at 4 DAI (red) or at 10 DAI (green; Supplementary Table 9). Biological networks of significantly enriched (P ≤ 0.05) Gene Ontology (GO) terms of P. infestans genes upregulated at 4 and 10 DAI (B), downregulated at 4 and 10 DAI (C), downregulated at 4 DAI (D), upregulated at 10 DAI (E) and downregulated at 10 DAI (F) were identified using the BiNGO tool and visualized with Cytoscape software. The color scale legend indicates the level of significance for enriched GO terms and white nodes indicate not significantly overrepresented categories. Dotted lines indicate connection between biological process categories in the GO chart, where ancestor and child are omitted for simplicity. No significant GO enrichment was found for P. infestans genes upregulated at 4 DAI.