Figure 2.

Venn diagram of the protein groups identified by the mass spectrometry proteomic analysis and the subsequent group comparisons. For the groups of proteins that were significantly different between FWT vs MWT, FWT vs FKO and MWT vs MKO animals (t-test p<0.05), the most significant KEGG pathways and reactome pathways for network enrichment in STRING interaction network analysis are listed. Additionally, 2-way ANOVA significance results of the NSAF quantification are presented for the commonly differing proteins (as initially identified by t-test) in both male and female WT vs KO: The spiked symbol indicates the 3 out of the 12 proteins that were not significantly different between sexes. n/s for not significant (p>0.05), *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001.