FIGURE 4.

Performance of RPA-Cas12a for the detection of Y. pestis-spiked environmental and tissue samples. Results for the mouse blood samples (A), lung tissues (B), and soil samples (C) spiked with Y. pestis ranging from 10¹ to 10⁷ CFU. For preparation of the spiked samples, 100 μl of mouse blood, 100 μl of lung homogenate (containing 20 mg of mouse lung tissues), and 100 mg of soil were used as the sample matrix. Real-time curve shows the fluorescence signals taken every minute within 1 h. Bar graphs show the endpoint fluorescence intensities detected at 1 h of detection. Data are represented as mean with SD (n = 3). RPA, recombinase polymerase amplification.