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. 2021 Jul 7;9:707970. doi: 10.3389/fcell.2021.707970

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Copyright © 2021 Dong, Liu, Deng, Shao, Tian, Chen, Huang, Lin, Chen and Shen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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C1GALT1 promotes LUAD cell migration and invasion in vitro. (A,B) The effect of C1GALT1 on cell migration was measured by wound-healing assay (A) and Transwell migration assay (B). (C) The effect of C1GALT1 on cell invasion was determined by Matrigel invasion assay. (D) Analysis of C1GALT1 expression by Western blot after treatment with itraconazole (ITZ) for 48 h. (E,F) The effect of itraconazole (2 μM) on cell migration and invasion was assessed by Transwell migration assay (E) and Matrigel invasion assay (F). Mock, cells transfected with empty plasmid; OV, cells transfected with C1GALT1 overexpression plasmid; shNC, cells infected with negative control shRNA; shRNAs, cells infected with C1GALT1 shRNA. Data are expressed as mean ± SD. *p < 0.05; **p < 0.001.