Fig. 2. DJ-1's deglycase function can be leveraged to generate a reproducible readout to evaluate potential modulators. (A) Scheme showing the role of DJ-1 in protein deglycation, as well as detection of the lactate product by a commercial lactate detection assay. (B) Lactate assay signal/noise determination of detected absorbance against time after commercial assay mix addition. (C) Characterization of various isatin analogs as DJ-1 inhibitors using lactate assay. Normalized endpoint absorbance vs. log[cpd] (μM) (D) structures of compounds tested in (C).