Fig. 4. Kinetic fluorescent assay to quantitate DJ-1 esterase activity. (A) 6,8-Difluoro-4-methylumbelliferyl acetate (7, DiFMUAc) substrate and fluorescent phenolate. (B) Michaelis–Menten analysis to determine substrate/enzyme parameters for DIFMUAc. N = 2. (C) Inhibition of DJ-1 following a 60 min pre-incubation with the compounds noted.
