Probing the SAR of DJ-1 using isatin-based analogs.

Compound	R1	R2	R3	R4	IC50 (μM)
1	CH2CH2Ph	F	H	H	0.118
2	CH2CH2Ph	Br	H	Br	0.0626
3	CH2Ph–p-OMe	Br	H	Br	0.267
4	CH2Ph–p-Me	Br	H	Br	0.676
5	CH2Ph–p-CF3	Br	H	Br	2.41
11	CH2Me	F	H	H	0.118
12	CH2C(C2H4)Ph	F	H	H	0.356
13	CH2CH2C(Me)3	F	H	H	0.851
14	CH2CH2BzImzMe	F	H	H	NA
15	CH2CH2Ph	OMe	H	H	2.93
16	CH2CH2Ph	COOH	H	H	9.79
17	CH2CH2Ph	CH2Ph	H	H	NA
18	CH2Me	CH2N4	H	H	NA
19	CH2CH2Ph	H	CH2COOH	H	2.35
20	CH2CH2Ph	H	H	H	0.701
21	CH2CH2Ph	F	H	OMe	0.0546