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Tyr residues proceeding the Pro in the CDR H3s of antibodies 4E10 and VRC42.01 replicated the SEC anomaly observed with 10E8v4. (A) Aligned sequences of CDR H3s of 10E8v4, 4E10 and VRC42.01 variants. Substituted amino acids were highlighted in red. (B) SEC profiles of 4E10, VRC42.01 and 10E8v4 variants along with the SEC profile of VRC01 control.
