Figure 1.

(A) Microscopic image showing large multinucleated giant cells (indicated by arrows) surrounded by multiple normal cells in an HSV-infected patient. The cells were stained with methylene blue, taken using an optical brightfield microscope [45]. (B) Images showing Vero/hSLAM cells coinfected by MeV and HSV-1 from different passages and a cell control (3rd, 6th, 10th, CC). As indicated in the 2nd and 3rd columns, the cells were subjected to immunostaining using antibodies against MeV M (green) and HSV-1 ICP0 (red) protein, and images were captured using laser scanning confocal microscopy at 40× magnification using a Leica TCS SP8 confocal microscope (Leica Microsystems; Wetzlar, Germany). The cell nuclei were stained with DAPI [68].