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. Author manuscript; available in PMC: 2022 Jul 6.
Published in final edited form as: Circulation. 2021 Jun 3;144(1):52–73. doi: 10.1161/CIRCULATIONAHA.120.047978

Figure 2. Higher levels of BMPR2 promoter methylation is associated with lower BMPR2 and SIN3a expression in hPASMC.

Figure 2.

A. BMPR2 (left) mRNA and protein (right) expression of indicated proteins were analyzed by RT-qPCR and immunoblotting in hPASMC and hPAECs. A representative blot is shown. GAPDH was used as a loading control. B. Representative images of Sin3a (Red) and α-SMA (Green) co-immunostaining in lung sections of non-PAH controls and iPAH patients. Nuclei were stained with DAPI (blue). C. Methylation level of the BMPR2 promoter region was analyzed by MS-PCR in hPASMC and hPAECs. Data are presented as mean ±SEM; ns=not significant, ** = p<0.01, *** P < 0.001.