Figure 4.

UPS transcripts accumulated in various growth conditions. (A) High temperature increased UPS level. Total RNA was extracted from wild-type cells cultivated overnight to exponential phase in YPD at indicated temperatures. UPS level was quantified as in Figure 1C. Error bars correspond to the standard deviation of three independent cultures. (B) UPS accumulated in post-diauxic and stationary (STAT) wild-type cells. Cells were grown in YPD at 30 °C overnight to the exponential phase (EXPO) and then left for either an additional 24 h or 11 days. RNA extraction and experimentation was performed as in Figure 1C, data were normalized by scR1 RNA reverse transcribed with specific primer (scR1-R),and quantified with an scR1F/R couple. scR1-normalized values were represented as a ratio of WT exponential values. Error bars correspond to the standard deviation of three independent cultures.