Figure 1.

RyR2 affinity-purification coupled to mass spectrometry. (A). Schematic showing the major methodological steps. LC = liquid chromatography; MS/MS = tandem mass spectrometry (B). Western blot validation of the immunoprecipitation of RyR2 from WT, RyR2 S2814A, and S2814D mouse ventricles. (C). Coomassie staining of gel containing samples from the various immunoprecipitations. Each lane (except for the input lane) is cut into ten equal pieces for in-gel digestion and LC-MS/MS analysis in parallel.