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. 2021 Jul 16;4(3):49. doi: 10.3390/mps4030049

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Overview of HiChIP and Hi-C data output. (a) Low-scale sequencing runs can aid in accurately estimating the quality of the prepared DNA libraries. Here, the Hi-C heatmap of murine thymocytes revealed the high-order chromatin organization even at a very low sequencing depth. The sample visualized had only 2.5 million total sequencing reads. (b) The low-scale sequencing runs highly correlated with the final deep sequencing. (c) The optimized protocol yielded a very low portion of short-range (<20 kbp) interactions while providing similar numbers of long-range cis and trans interactions. The comparison to HiChIP libraries produced by the group developing the original HiChIP protocol is provided.