Table 1.

Composition of the Fill-in Master Mix.

Reagent	Volume Per Reaction
10× NEBuffer 2 (NEB, M0210L)	30 μL
ddH2O	238.5 μL
1 mM Biotin-16-dCTP (Jena Bioscience, NU-809-BIO16-L)	15 μL
10 mM dATP (Promega, U1240)	1.5 μL
10 mM dGTP (Promega, U1240)	1.5 μL
10 mM dTTP (Promega, U1240)	1.5 μL
5 U/μL DNA Polymerase I, Klenow Fragment (NEB, M0210L)	12 μL