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. 2021 Jul 19;4(3):50. doi: 10.3390/mps4030050

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Expression of pluripotency markers in hiPSCs. (A) Phase contrast images and ICC for pluripotency markers SSEA-4, OCT3/4, NANOG, and Tra-1-60, with Ho342 nucleic acid counterstain. (B) Quantification of OCT3/4-positive cells or SSEA-4-positive cells shown in (A). (C) qPCR for mRNA expression of pluripotency genes POU5F1, NANOG, c-MYC, KLF4, SOX2, and ZFP42 in hiPSCs compared to H9 ESCs. mRNA expression in H9 was set as 1.0. The mean and SD are from technical triplicates from three independent experiments.