Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jul 19;4(3):50. doi: 10.3390/mps4030050

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Differentiation of hiPSCs into three germ layers. (A) Representative phase contrast images of EB formation (left) and differentiation into three germ layers by ICC with the ectoderm (PAX6), mesoderm (SMA), and endoderm (VIM) markers (indicated above images). Nuclei are counterstained with Ho342. (B) qPCR for mRNA expression of three germ layer genes. Quantification of expression of the ectoderm (PAX6, NCAM1), mesoderm (MIXL1), and endoderm (VIM) markers in hiPSCs compared to H9 ESC. mRNA expression in H9 was set as 1.0. The mean and SD are from technical triplicates from three independent experiments.