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. 2021 Jul 19;4(3):50. doi: 10.3390/mps4030050

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Differentiation of hiPSCs into forebrain cortical neurons. (A) Schematic representation of the in vitro differentiation protocol used to generate cortical neurons from hiPSCs. Boxes indicate differentiation state. This protocol was performed in 11 independent lines, with all lines performing similarly. (B) qPCR for mRNA expression levels of neuronal markers MAP2, NCAM1, and TUBB3 in NPC and 4-week-old differentiated cortical neurons compared to IPSCs. mRNA expression in iPSCs was set as 1.0. The mean and SD are from technical triplicates from two independent experiments. (C) Expression of cortical layer markers FOXP1 and SATB2 by qPCR analysis of 4-week-old differentiated cortical neurons compared to iPSCs. mRNA expression in iPSCs was set as 1.0. The mean and SD are from technical triplicates from two independent experiments.