Table 2.

Twenty-nine defined biomarkers for fibrosis: In the first column, gene symbols are given followed by amino acid sequences. Also listed are the adjusted p-values; mean peptide intensities (normalized using internal standards) in fibrosis (IFTA ≥ 15%) and no fibrosis (IFTA < 10%) groups are given with fold change (fibrosis/no fibrosis) for the training cohort 100/100. In addition, the unadjusted Wilcoxon p-values, mean intensities, and fold change for the etiology-matched cohort are given (55/55). * This peptide could not be validated in the etiology-matched cohort (significant regulation in opposite direction).

Urinary Peptides	Training Cohort 100/100					Etiology Matched Cohort 55/55
Gene Symbol	Sequence	adj. p-Value (BH)	Mean Intensity Fibrosis	Mean Intensity No Fibrosis	Fold Change	Unaj.Wilcox-p-Value	Mean Intensity Fibrosis	Mean No Fibrosis	Fold Change
COL10A1	GHPGPSGPPGKpGYGSpGLQGEpGLPGPPGPS	2.42 × 10−4	782.77	380.77	2.056	1.34 × 10−2	755.71	443.15	1.705
COL1A2	GPQGVQGGKGEQGPPGPPGFQGLPGPSGpAGEVGKpGERG	2.42 × 10−4	1151.22	272.13	4.230	3.21 × 10−3	1108.09	328.9	3.369
COL1A2	DQGPVGRTGEVGAVGpPGFAGEKGPSGEAGTAGPpGTpGP	8.56 × 10−4	196.08	75.14	2.610	1.22 × 10−2	168.37	83.85	2.008
AHSG	SLGSPSGEVSHPRKT	8.72 × 10−4	2282.82	975.54	2.340	4.44 × 10−4	2515.71	1480.13	1.7
AHSG	VVSLGSPSGEVSHPRKT	9.37 × 10−3	11,404.37	7829.98	1.457	3.12 × 10−2	13,131.49	12,697.43	1.034
PIGR	LFAEEKAVADTRDQADGSRASVDSGSSEEQGGSSRA	1.22 × 10−2	774.23	654.63	1.183	2.49 × 10−3	624.32	360.05	1.734
COL1A2	VGRTGEVGAVGPpGFAGEKGPSGEAGTAGPpGTpGP	1.82 × 10−2	109.41	46.41	2.357	2.91 × 10−1	92.33	59.3	1.557
COL3A1	ARGLpGppGSNGNPGPPGPSGSPGKDGPPGPAGNTGAPG	2.34 × 10−2	902.6	594.85	1.517	1.01 × 10−1	889.74	771.39	1.153
SERPINC1	FSPEKSKLPGIVAEGRDDLYVSDAFHKAF	2.34 × 10−2	9438.98	5838.02	1.617	1.80 × 10−3	11,768.37	8101.28	1.453
COL2A1	GETGAAGpPGpAGPAGERGEQGAPGP	2.34 × 10−2	43.02	135	0.319	1.03 × 10−2	58.08	160.81	0.361
COL4A1	pGIPGFPGSKGEMGVMGTPGQPGSPGPVGAPGLPGEKGDH	2.34 × 10−2	3045.2	1456.47	2.091	4.89 × 10−2	2563.4	1629	1.574
COL1A1	ANGApGNDGAKGDAGApGApGSQGApGLQGMpGERGAAGLPGp	2.69 × 10−2	1210.38	814.9	1.485	1.94 × 10−1	1267.81	1012.08	1.253
COL3A1	ApGPAGSRGApGPQGpRGDKGETGERG	2.69 × 10−2	1103.52	618.43	1.784	1.27 × 10−1	857.9	692.61	1.239
COL1A1	GADGQPGAKGEpGDAGAKGDAGPpGPAGP	2.69 × 10−2	108.1	388.09	0.279	7.21 × 10−3	106.07	519.75	0.204
COL1A1	ANGApGNDGAKGDAGApGApGSQGApGLQGMpGERGAAGLpGp	2.74 × 10−2	447.16	170.81	2.618	2.04 × 10−1	470.53	214.82	2.19
HBA1	AAHLPAEFTPAVHASLDKFL	2.81 × 10−2	610.19	14,067.56	0.043	9.65 × 10−3	903.23	19,261.46	0.047
COL1A1	ADGQpGAKGEpGDAGAKGDAGPPGPAGP	2.81 × 10−2	212.86	365.65	0.582	1.01 × 10−1	217.32	302.79	0.718
COL3A1	EGGKGAAGpPGPpGAAGTpGLQG	2.81 × 10−2	689.84	500.7	1.378	7.94 × 10−2	705.78	551.83	1.279
COL22A1	GTEGKKGEAGPPGLPGPpGIAGpQGSQGERGADGEVGQKGDQGHPGVPGFMGPPGNPGP	2.81 × 10−2	192.19	159.22	1.207	4.74 × 10−2	171.5	166.83	1.028
AHSG	GVVSLGSPSGEVSHPRKT	2.81 × 10−2	2476.64	1429.55	1.732	2.24 × 10−2	2876.18	2229.17	1.29
PIGR	FAEEKAVADTRDQADGSRASVDSGSSEEQGGSSRALVSTLVPL	3.06 × 10−2	891.67	377.71	2.361	3.43 × 10−2	861.08	320.08	2.69
COL2A1	ppGSNGNpGPPGPPGPSGKDGPKGARGDSGPPGRAGEPG	3.50 × 10−2	412.14	184.53	2.233	2.54 × 10−1	396.08	243.4	1.627
COL18A1	DDILASPPRLPEPQPYPGAPHHSS	3.77 × 10−2	611.67	433.29	1.412	5.12 × 10−1	560.24	524.1	1.069
COL3A1	EpGRDGVpGGPGm	3.77 × 10−2	2254.17	1608.12	1.402	1.08 × 10−2	2160.1	1392.93	1.551
HBA1	AAHLPAEFTPAVHASLDKFLAS	4.15 × 10−2	847.52	30,583.66	0.028	3.06 × 10−2	1046.13	30,595.91	0.034
FGA	DEAGSEADHEGTHSTKRGHAKSRPV	4.15 × 10−2	31,926.35	22,421.31	1.424	5.01 × 10−1	29,485.54	34,133.85	0.864
AHSG	VSLGSPSGEVSHPRKT	4.15 × 10−2	3680.2	2187.3	1.683	2.25 × 10−2 *	2326.63	3525.8	0.66 *
COL3A1	GpGSDGKPGPpG	4.86 × 10−2	145.26	347.21	0.418	2.28 × 10−2	192.46	399.37	0.482
COL1A1	GSpGSpGPDGKTGPPGPAG	4.86 × 10−2	74.29	178.76	0.416	4.19 × 10−2	68.72	157.74	0.436