Fig. 2.

Hyperbiofilm phenotype is caused by rpoS mutation. rpoS point mutation, full deletion (A) and regions deletion strains (B) were increased the biofilm formation. The biofilm formation by the indicated strains was displayed with CFU of biofilm cells on 5 mm glass bead. Data are presented as the mean ± s.d. of four biological replicates. Significance was determined using a Student’s t test: ***P < 0.001. EV represents the empty vector pHERD20T in this assay